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Msg  Description
# 4986 SCI.Response to Brad [Mike Darwin]
  First, we have done experiments with 24 hours of flush-store on ice using dogs . . . our surprise (and mixed pleasure/displeasure) the brains using our solution looked much better than the Viaspan brains. The principal differences were massive swelling of . . . control (vitally perfused-fixed dogs) at 24 hours. Brad goes on to say: >I don' . . . by Thomas Donaldson on conservation of memory after ischemic insult. I had high hopes for . . . is the story of Hossman's one hour ischemic cat which survived and recovered (walked . . . one sitting on my lap (cats do, after all, look down on people). Finally they . . . with fixative. They then looked at various brain areas. There were heavy neuron losses across . . . thin skulls and you can see the brain through them. If you reflect the skin over a mouse's brain and repeatedly over a period of weeks . . . love 'em anyway) and have barely learned, after all this time (a year) that the . . . of the best physicians in the United States to deal with various aspects of managing
(14 Oct 95 16:53:36, 16 KB)
# 9139 Cryonics & organ donors [Stephen W. Bridge]
  provide reasonable preservation for a member's brain is strongly dependent on how fast our . . . protective chemicals. Forcing Alcor to wait several hours while surgeons remove a heart and kidneys is not good for your brain. We assume you are involved in cryonics at all because you want your brain treated with utmost care. 2. All states require evidence of "brain death" before a hospital can remove organs from a donor. "Brain death" typically means that the brain has had no circulation for 24 hours (not at all good for your brain) . . . matching is especially difficult in the United States, with the nearly infinite mix of racial . . . circulatory system. Rapid perfusion of the brain after the head has been separated from the
(Tue, 10 Feb 1998, 7 KB)
# 14802 complete cryonic suspension/revival demonstrated [Brent Thomas]
  loaded systems (i.e. you and me after alcor is finished with us) do not? . . . trying to load the system with it after death? here is an interesting article about . . . hoping they diffuse correctly into the cells after death its much much better to ALTER . . . still very much alive. They thaw out after about 20 minutes at room temperature, sit . . . of preserving the whole body, head, or brain of persons recently declared legally dead, in . . . for transplant can last six to eight hours after death, a heart and lung only four to five hours. If, by freezing organs, doctors could avoid . . . longer. "These animals are living in a state of suspended animation, and if you think
(Mon, 30 Oct 2000, 10 KB)
# 17001 Brains liquefy? [Mike Perry]
  From: Mike Perry <mike@alcor.org> Subject: Brains liquefy? >From: Thomas Donaldson <73647.1215@compuserve. . . . A short comment for Mike Perry: > >No, brains do not liquefy shortly after death. Or even some time after >death. Well, "short" is a relative term . . . me, about two years ago, that the brain will liquefy if not treated. Not in . . . in the sense of entering a liquefied state. Of course, eventually decomposition will reduce it to essentially nothing, regardless of what states it passes through in the meantime, which . . . to sound overly pessimistic. Up to 30 hours postmortem, maybe more, the largely untreated brain
(Mon, 16 Jul 2001, 2 KB)
# 5858 SCI.ukrainian research [Pichugin]
  between 10 and 20 deg C per hour. One was frozen to - 80 C, the . . . the work with glycerolized sheep heads and brain slices. ----------- 5 Feb. 1996 After cryopreservation of sheep heads last year by . . . results, I got the idea of using brain slice cultures with the aim of reanimation of cryopreserved brain tissues, using measurement of BEA [bioelectric activity] . . . method of examination of viability of the brain tissues [since there was considerable disagreement on . . . have been published in The Immortalist. Rabbit brain pieces were frozen to - 196 C; after thawing, washout of glycerol, and reanimation in . . . but that study did not show the state of synapses and integral BEA of neural
(Fri, 1 Mar 1996, 13 KB)
# 9621 21CM Cerebral Resuscitation [Mike Darwin]
  under a freeway underpass in the United States became aware that the FDA had approved . . . system which had not been ravaged by hours of pre-mortem shock or multi-system . . . went into rigor mortis within a few hours despite excellent mechanical CPR, buffering, anticoagulation, and . . . was that it took him nearly 8-hours to cool to just about room temperature! . . . patient having gone into rigor within 2 hours after the start of "transport." Watching cryopatients die . . . long-term recovery of dogs (and people) after periods of cardiac arrest of much greater . . . being detection of free radical inhibition in brain tissue upon reperfusion by a measurement such
(Tue, 5 May 1998, 16 KB)
# 29650 newsweek on low temps to save heart attack vics [un person]
  always with precision. He wasn't yet "brain dead," implying a permanent cessation of cerebral . . . the hundred billion neurons in Bondar's brain would have used up their residual oxygen, . . . 000 times a year in the United States, someone's heart stops beating on the . . . his death near his New Jersey home. After being discovered by his wife, unconscious and . . . heart stops: about new research into how brain cells die and how something as simple . . . always been taught in medical school: that after about five minutes without a pulse, the brain starts dying, followed by heart muscle the . . . that can play out over the succeeding hours, or even days. Dying turns out to
(Mon, 16 Jul 2007, 14 KB)
# 12464 21CM Patent: Some answers and questions [Mike Darwin]
  of new variations and applications." Donaldson also states: "There is also some question in my . . . or completely glassy or otherwise non-crystalline state. Freezing is a workable method for cryopreservation . . . human cryopreservvaion) had a client who's brain had been fixed in formlain and been . . . anamolously low concentrations, it penetrated the entire brain rapidly and uniformly, including the myleinated axons which have been a major barrier to successful brain cryoprservation, and it passed my bioassay tests, . . . 21CM for nearly two years -- and considerably after the first disclosure document for the patent . . . pivotal contributions and spent countless back-breaking hours in the laboratory. Ettinger continues: >Naturally, we
(Tue, 28 Sep 1999, 21 KB)
# 8723 Re: CryoNet #8708 - #8716 [Thomas Donaldson]
  of cryonicists remained at its present low state for centuries. We advocate freezing people now . . . suspension: just why we freeze pieces of brain obtained from the coroner, or freeze people found only hours after their "death", or all the other acts
(Wed, 5 Nov 1997, 6 KB)
# 4927 CryoNet #4921 - #4925 [Mike Darwin]
  sometime now) which is about a 2 hour drive from us in Rancho Cucamonga. Joe . . . come out regularly to participate in our brain resuscitation and cryopreservation research: often staying all . . . at little or no cost. Straight freezing brains is easy, and rodents are, for now . . . When CI or I have straight frozen brains what we get back after thawing is hash. If you try to look at straight frozen brains in the frozen state with freeze substitution you can't tell
(28 Sep 95 13:51:44, 8 KB)

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